Hello, Separate (Repeated) acquisitions will always have gap between them. The duration of this depends on many things, system performance, time to export to csv, opened views, measurements... I see about 5-10ms delay between acquisitions with WaveForms with the attached Script. With a custom application you might get a bit better result. After installing WaveForms 2015 see C:\Program Files (x86)\Digilent\WaveFormsSDK\samples\py With Record mode (select instead Repeated option) you