Hi @kraiskil I suspect the ferrites (50mOhm/1A BK2125HS470-T) on USB GND and VCC got damaged or weekend on your board. These are below the USB plug, on the edge of the bottom side, and are intended for noise filtering, but also act as a fuse to protect the computer beside D28, in case of connecting the device ground to high external voltage. https://reference.digilentinc.com/reference/instrumentation/analog-discovery-2/reference-manual#usb_power_control